Heat shock protein 90 (HSP90) is a critical molecular chaperone that plays a significant role in the cellular stress response. This protein helps stabilize misfolded or denatured proteins, which is essential for maintaining cellular homeostasis. Many proteins that interact with HSP90 are important to oncogenic processes, influencing several key hallmarks of cancer.
The inhibition of HSP90 represents a promising strategy for therapeutic intervention against cancer. HSP90 inhibitors, which target the ATP binding pocket on the N-terminal domain, can lead to the degradation of HSP90 client proteins via the ubiquitin-proteasome pathway. Notable natural products such as geldanamycin and radicicol inhibit the intrinsic ATPase activity of HSP90, thereby promoting the degradation of oncogenic proteins. Targeting HSP90 thus provides a valuable pharmacological basis for developing new anticancer therapies.
Our Expertise
Creative Bioarray leads in drug discovery services, offering expert HSP90 screening. Our capabilities include conducting high-throughput screening (HTS) assays designed to identify HSP90 inhibitors from extensive chemical libraries. We employ various specialized assays to evaluate compounds based on their ability to:
- Inhibit HSP90-catalyzed ATP hydrolysis
- Prevent HSP90-mediated refolding of denatured proteins, such as luciferase
- Competitively inhibit the binding of ligands to HSP90’s ATP binding domain
- Deplete cultured cells of HSP90 client proteins
These targeted screenings have led to the discovery of a multitude of potential HSP90 inhibitors.
Comprehensive Support
Our extensive services portfolio ensures optimal support throughout the screening process. Our team of experts provides assistance by facilitating:
- Target and inhibitor screening using our HSP90α and HSP90β assays
- IC50 determination and evaluation at various concentrations
- Follow-up studies using in-house produced proteins
- Prompt responses to inquiries and guidance for your projects
Creative Bioarray is committed to delivering comprehensive services and high-quality results in HSP90 screening and profiling. If specific requirements exist for HSP90 inhibitor screening, we encourage you to reach out to us. We look forward to collaborating on your exciting projects.
